Protocols

Moxi GO & Moxi GO II

Nuclei or Nucleated Cell Counting Protocol with Coulter Principle and Propidium Iodide

Step by step instructions for antibody labeling and detection of cell surface markers with the Moxi GO II System

Step by step instructions for intracellular immunolabeling of peripheral blood using the Moxi GO II system.

Step by step instructions for cell surface immunolabeling of peripheral blood using the Moxi GO II system.

Step by step instructions for staining with Calcein-AM and PI using the Moxi GO II system.

Step by step instructions for staining with FITC- Annexin V and PI using the Moxi GO II system.

Step by step instructions for staining with AO and PI using the Moxi GO II system.

Protocol for use of the Moxi Cyte Apoptosis Kit (MXA071) with the Moxi GO II system

Step by step protocol for processing whole blood for measuring mitochondrial potential using TMRE on the Moxi GO II instrument.

Step by step protocol for processing whole blood for ROS detection on the Moxi GO II instrument.

Step-by-step procedure for swapping the 561nm/LP and 650nm/LP filters on the Moxi GO II

Protocol for immunolabeling whole blood samples with a no-wash (no centrifugation) lysis buffer. For use with the Moxi GO

FOR RESEARCH USE ONLY on the Moxi Flow or Moxi GO Instruments

Step by step protocol for fixing cells in Ethanol.

Step by step protocol for antibody labeling of intracellular markers for subsequent analysis on the Moxi GO and Moxi Flow instrument.

Step by step protocol for processing whole blood for cell surface immunolabeling and subsequent detection on the Moxi Flow and Moxi GO instruments.

for Orflo Cytometers

for use with the Moxi Flow or Moxi GO

Moxi V

Protocol for staining cells with Propidium Iodide (PI) for Viability Assessment

Rapid Protocol for determining the total nucleated count (TNC) in RBC contaminated samples

Protocol for using MXA028 check beads to verify the performance of the Moxi V system

Moxi Flow

FOR RESEARCH USE ONLY on the Moxi Flow or Moxi GO Instruments

for Orflo Cytometers

Step by step protocol with pictures of how to use the MF-S Flow sensor cassette to obtain WBC counts from whole blood using a nucleated cell counting approach.

Step by step instructions for staining with Annexin V using the Moxi Flow device.

Step by step protocol for antibody labeling of cell surface markers for subsequent analysis on the Moxi Flow instrument.

Step by step guide demonstrating how to run a system check using calibrated bead sets with the Moxi Flow device and MF-M or MF-S flow sensor cassettes.

Step by step protocol for antibody labeling of intracellular markers for subsequent analysis on the Moxi GO and Moxi Flow instrument.

Step by step protocol for fixing cells in Ethanol.

Step by step protocol for processing whole blood for cell surface immunolabeling and subsequent detection on the Moxi Flow and Moxi GO instruments.

Moxi Z

Step by step guide demonstrating how to run a system check using calibrated bead sets with the Moxi Z device and Type S or Type Mcassettes.

Step by step instructions for staining with Annexin V (Apoptosis) and PI (Viability) using the Moxi Flow device.

Step by step protocol for collecting a whole blood specimen and preparing a buffy coat cell suspension for later analysis in the Moxi Flow or Moxi Z devices

Step by step protocol for preparing mammalian cells for use in the Moxi Z device