Cell Counting Cassettes
Products
Moxi Flow Cassettes, Type M+, Case
Product Code: MXC042
Moxi Flow Cassettes, Type M+, 10 Pack Box, 25 Flow Sensor Cassettes/Pack (500 Tests)
Our Price: $1,980.00
Discounts could be available with our subscription service.
Higher Performance & Simplicity in One Disposable Cassette.
Patent-protected thin-film cassettes provide highly accurate, repeatable cell counts and cell size analysis in under 8 seconds (15 seconds for Type S+ cassette). Inspired by the gold-standard Coulter Principle used in high-end counting systems worldwide, the Moxi cassette flows thousands of cells per test through a cell sensing zone to accurately capture the impedance-based volumetric measurement of each individual cell in the sample. Impedance-based cell counting of individual cells is proven to be over 95% accurate versus a 75% accuracy with image-based cell counting methods.
No system contamination and integrated pre-filter for clogging prevention.
Moxi cassettes are designed with a unique “”cell sieve”” to minimize cell clumping and clogging. In addition, Moxi cassettes contain 100% of your sample within the cassette body, eliminating the possibility for system contamination and sterilization. Cassettes are simply and easily discarded when testing is finished.
Two tests per cassette.
Each Moxi cassette offers two individual tests. The system will alert the user if a used cassette is mistakenly inserted in the unit.
Multiple cassette options.
Users can choose from Moxi Type M+ or Moxi Type S+ cassettes depending on cell specifications
Part Number | MXC042 |
Cassette Types | Type M+ |
Cell Particle Concentration Range | 10,000 – 750,000 cells/mL |
Cell Types Tested | HEK-293|HeLa|PC12|CD3+T|CHO-K1|Cos-7|HepG2|Hybridoma|Jurkat E6-1|K562|MCF7|Mesenchymal SC|Monocyte|Mouse ESC| NIH 3T3| PBMC (cultured)|Red Blood Cells (RBC)|L5178y| C. albicans (Yeast)| S. cerevisiae Vin 13 (Yeast)|S. cerevisiae X5 (Yeast)|Wine Yeast (natural fermentaion)|S.cerevisiae (Baker’s Yeast|Safale US-05 Yeast| |
Compatible Instruments | Moxi Flow|Moxi GOII|Moxi V |
Intended Use Statement | For Research Use Only. Product is not for use in diagnostic procedures |
Measurable Dynamic Range | 4 – 35 microns |
Measurement Time | 10 seconds |
MPI Cell Health Ratio | Size Histogram Only |
Number of Tests | 500 |
Overall Dimensions | 260 L x 125 W x 53 H (mm) |
Sample Type | Cell Preparations |
Sample Volume | 60 µL |
Sterility | Non-sterile |
Measurable Cell Volume | 34 – 22,449 fL |
Weight | 657.7 g |
How It Works
The operating principle behind the Moxi Flow Smart Cell Analyzer is a unique combination of Coulter-style cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using a 532nm solid state diode laser. Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is easily performed on the unit using a interactive touch display, and the resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analyzed.
Step 1:
Select desired app, insert the cassette and close the doors.
Step 2:
Once auto-alignment is complete, open the top door and pipette 60μl of labelled sample into the cassette.
Step 2:
Close the top door, assays run automatically and results are generated in ~10 seconds. Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into Moxi Flow, and load Sample 2.
Data:
Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any Cell Analysis package. The actual Moxi Flow screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi flow and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.